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Temperature
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Incubation time with dsRNA against Mlig-ddx39 gene until lethalitya, days, mean ± standard deviation
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Percentage of animals with enlarged testesb after 4 days of incubation with dsRNA against Mlig-sperm1 gene
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DV1
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NL10
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NL10
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20 °C
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17.8 ± 1.3
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16.6 ± 1.9
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0%
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25 °C
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10.1 ± 0.6
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9.5 ± 0.5
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25%
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30 °C
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7.9 ± 0.2
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7.2 ± 0.4
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85%
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- ano lethality was observed at the tested temperatures when incubating animals with dsRNA against gfp as a control
- bwhen testes touch each other